Detection

CHROMOGENS

We offer a large variety of IHC-specific permanent chromogens, for use with the two most commonly used enzyme labels: Horseradish Peroxidase (HRP) and Alkaline Phosphatase (AP). Chromogens are chemical compounds used within IHC staining procedures that produce a colored end-product that can be visualized under bright-field microscopy.

Biocare’s chromogens generate a broad spectrum of colors, to find out more click on the button below.

Chromogens

MACH DETECTION KITS

Biotin-free micro-polymer technology
Superior antigen access
Unsurpassed specificity and sensitivity
Greater primary antibody dilutions
Minimal background staining

To find out more on Biocare's detection solutions click on the button below.

Detection

MACH 4™

MACH 4 is a highly sensitive universal detection system. The MACH 4 system can detect either mouse or rabbit antibodies, and is a two-step universal detection method. MACH 4 is available in a universal (anti-mouse and anti-rabbit) format, labeled with either Horseradish Peroxidase (HRP) or Alkaline Phosphatase (AP). MACH 4 detection consists of two reagents: the secondary, also known as the enhancing reagent, is applied between the primary antibody and micro-polymer reagent. MACH 4 is ideal for use with antibody solutions prepared from concentrates or prediluted antibody solutions. Due to its increased sensitivity, MACH 4 enables a significantly higher dilution of concentrated antibodies compared to other polymer-based detection systems.

Enhanced probe to increase sensitivity 10- to 20- fold
Increased density of enzymes bound to tertiaries\
10 – 20 times more sensitive than conventional dextran polymer systems
20 – 40 times more sensitive for nuclear staining
Suitable to detect mouse specific anti-biotin or antidigoxigenin antibody in in situ hybridization (ISH)
Micro-polymer allows superior specificity/sensitivity, nuclear access and primary antibody dilution
Compatible with automated immunostainers
Compact structure enables 40-fold increase in primary antibody dilution.

MACH 3™

MACH 3 is a two-step, biotin-free detection system which provides excellent specificity, sensitivity and nuclear staining for mouse or rabbit primary antibodies. Biocare offers MACH 3 Detection Kits for either mouse or rabbit primary antibodies, labeled with either Horseradish Peroxidase (HRP) or Alkaline Phosphatase (AP).

A sensitivity-enhancing secondary reagent
Increased density of enzymes bound to tertiary target
5- to 10- fold increased staining sensitivity when compared to conventional dextran polymer detection kits
Superior staining for nuclear and cytoplasmic/cell surface antigens
Elimination of background
High primary antibody dilution significantly improves specificity and reduces cost
Adapted for all Biocare’s prediluted antibodies
Compatible with automated immunostainers
MACH 3’s superior sensitivity allows clean and strong staining, even at higher primary antibody dilution factors.

MACH 2™

MACH 2 Detection is a one-step / one-solution, biotin-free detection system which combines superior work flow and sensitivity for mouse and rabbit primary antibodies.

MACH 2 detection consists of a single reagent applied after the primary antibody. MACH 2 is ideal for use with prediluted antibodies or concentrates with equal success. MACH 2 may be used for 7-step multiplex stains with certain antibodies.

MACH 2 increases staining specificity compared to conventional dextran backbone polymer. MACH 2 is available in anti-mouse, anti-rabbit, and ‘universal’ (anti-mouse & anti-rabbit) formulations, labeled with either Horseradish Peroxidase (HRP) or Alkaline Phosphatase (AP).

Increase specificity 3-4 fold in nuclear staining & 2 fold in cytoplasmic/cell surface staining
Superior to dextran backbone polymers

MACH 1™

The MACH 1 Universal Polymer Detection system offers a cost-effective alternative method of polymer detection for IHC laboratories (not available in the U.S.). Specifically titered for Biocare prediluted antibodies, MACH 1 provides superior staining and a reduced protocol time as compared to the conventional ABC system.

This new two-step, biotin-free technology uses a specific probe to detect mouse antibodies. The probe is followed by an HRP-polymer that binds to both the probe and to rabbit antibodies. Time consuming avidin-biotin and normal serum blocking steps are eliminated. The innovative HRP-polymer technology provides superior staining for mouse and rabbit antibodies when compared to ABC, biotin-streptavidin (BSA) and other polymer detection kits.

Elimination of avidin-biotin and normal serum blocking steps
Reduce protocol length by approximately 90 minutes compared to ABC method
High value, cost-effective alternative to biotin-streptavidin or ABC detection
Eliminates endogenous non-specific background staining

Detection

CHROMOGENS

MACH DETECTION KITS

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