INTERSCIENCE NEWS: High-performance solution for real-time colony counts on filtration membranes in microbiological analysis with ScanStation

Check out the ScanStation study…

The enumeration of viable microorganisms on agar culture media remains the recommended reference method or im-posed by the various standards for microbiological tests in release control. It involves either direct […]

Sébastien BONOT and Gautier VARIN – APTARPHARMA
Manon LABORIE – INTERSCIENCE

(La Vague N°67, October 2020)

Read the full article on www.a3p.org: https://www.a3p.org/en/scanstation-microbiological-analysis/

ScanStation is a real-time incubator and colony counting station centralizing incubation, detection and counting of up to 300 Petri dishes simultaneously.

ScanStation 100

Real-time incubator and colony counter

100 Petri dishes

Ref. 439 100

ScanStation 100 is a unit centralizing incubation, detection and counting of Petri dishes. Colonies are detected & counted as soon as they appear. Follow the growth curve of the colonies. ScanStation 100 can accommodate up to 100 Petri dishes.

Capacity: up to 100 Petri dishes
Counting on pour plate, surface, Spiral, filtration membranes
Petri dish sizes: Ø 55 to 65 mm, Ø 90 mm
Accuracy of incubation temperature: ± 1°C
Reading time in between each dish: 30 min or 1 hour

Specifications/Videos/Downloads/Accessories: Interscience-ScanStation100

ScanStation 200

Real-time incubator and colony counter

200 Petri dishes

Ref. 439 200

ScanStation 200 is a unit centralizing incubation, detection and counting of Petri dishes. Colonies are detected & counted as soon as they appear, from the beginning of the incubation. Follow the growth curve of the colonies. ScanStation 200 can accommodate up to 200 Petri dishes.

Capacity: up to 200 Petri dishes
Counting on pour plate, surface, Spiral, filtration membranes
Petri dish sizes: Ø 55 to 65 mm, Ø 90 mm
Accuracy of incubation temperature: ± 1°C
Reading time in between each dish: 30 min or 1 hour

Specifications/Videos/Downloads/Accessories: Interscience-ScanStation200

ScanStation 300

Real-time incubator and colony counter

300 Petri dishes

Ref. 439 300

ScanStation 300 is a device centralizing incubation, detection and counting of Petri dishes. Colonies are detected & counted as soon as they appear. Follow the growth curve of the colonies. ScanStation 300 can accommodate up to 300 Petri dishes.

Capacity: up to 300 Petri dishes
Counting on pour plate, surface, Spiral, filtration membranes
Petri dish sizes: Ø 55 to 65 mm, Ø 90 mm
Accuracy of incubation temperature: ± 1°C
Reading time in between each dish: 1 hour

Specifications/Videos/Downloads/Accessories:Interscience-ScanStation300

Or for more information visit: Interscience-Real-time-incubator-and-colony-counter

SOX10

“Used as a concentrate, mAb clone BC34 gave an overall pass rate of 79% (38 of 48) of which 58% was optimal (see Table 1). As shown in Table 3, optimal results could be obtained on the three main fully automated IHC platforms from Dako, Leica and Ventana.”
(NordiQC, 2019)

Link to assessment: https://www.nordiqc.org/downloads/assessments/117_94.pdf

Link to Biocare: https://biocare.net/product/sox10-antibody/

p40

“mAb clone BC28 was by far the most commonly used and successful p40 antibody, giving pass rates of 86% and 91% for the concentrated and RTU formats.”
(NordiQC, 2016)

Link to assessment: https://www.nordiqc.org/downloads/assessments/83_10.pdf

Link to Biocare: https://biocare.net/product/p40-antibody/

p63

“The Ready-To-Use format of mAbs clones 4A4 and DAK-p63 performed slightly better compared to laboratory developed assays using the concentrated format.”
(NordiQC, 2016)

Link to assessment: https://www.nordiqc.org/downloads/assessments/83_62.pdf

Link to Biocare: https://biocare.net/product/p63-antibody/

Uroplakin II

“The concentrated format of mAb clone BC21, within a laboratory developed assay, was most successful and provided the highest proportion of optimal results.”
(NordiQC, 2020)

Link to assessment: https://www.nordiqc.org/downloads/assessments/134_112.pdf

Link to Biocare: https://biocare.net/product/uroplakin-ii-antibody/

NKX 3.1

“The widely used rmAb clone EP356 and pAb CP422 could both be used to provide an optimal result for NKX3.1.”
(NordiQC, 2020)

Link to assessment: https://www.nordiqc.org/downloads/assessments/131_100.pdf

Link to Biocare: https://biocare.net/product/nkx3-1-antibody/

SMAD4

“The RmAb clone EP618Y provided the highest proportion of sufficient and optimal results.”
(NordiQC, 2019)

Link to assessment: https://www.nordiqc.org/downloads/assessments/122_105.pdf

Link to Biocare: https://biocare.net/product/smad4-antibody/

Differentiating Diluents: Tricks of the Trade for Diluting Antibodies

Biocare Medical

Whitepaper – Differentiating Diluents: Tricks of the Trade for Diluting Antibodies

Aqueous environment greatly influences antibody conformations. When preparing working solutions of concentrated antibodies, it is important to select a diluent that will not alter the stability and binding properties of the antibody. Charge, hydrophobicity, and other physiological/chemical properties can affect antibody-diluent combinations.

Binding of antigens to antibodies is pH dependent and highly controlled by ionic interactions. Diluents with a relatively neutral pH (pH of 7.0- 7.4) are often ideal buffers and a great starting point for optimization. “The isoelectric point, or pI (the pH at which the net electric charge of a molecule is zero), for immunoglobulins can range from 5.8 to 8.5 for a given antibody.”1 If the pH of the diluent is too close to the pI of the antibody, solubility can weaken, resulting in negative effects on staining and background. Amino acid side chains in antibodies and antigens are also affected by pH.

Biocare Medical’s Revival Series of Super Antibody Diluents: DaVinci Green, Monet Blue, Renoir Red and Van Gogh Yellow are optimized formulations for improving antibody titers and are extremely stable for long-term storage.

To learn more about Biocare Medical’s Antibody Diluents, download the whitepaper below:

Biocare Medical – White Paper – Antibody Diluents

P40 ANTIBODY PROMOTION

PROMOTION: Exciting start to 2021 with our significantly discounted Biocare Medical p40 Antibody

p40 (M) [BC28]

The mouse monoclonal antibody p40 [BC28] recognizes an epitope unique to the p40 protein and may have applications in cases where p63 has traditionally been used. p63 [4A4] recognizes both the p63 and p40 proteins. As a result, p63 suffers from specificity limitations due to reactivity in a subset of lung adenocarcinomas (ADC). In contrast, p40 is selectively expressed in lung Squamous cell carcinoma (SqCC), offering an opportunity for improved specificity. p40 antibody (M) [BC28] recognizes an epitope unique to p40, which may result in diminished reactivity in lung ADC and increased specificity. Studies have supported routine use of p40 as an alternative for p63. In contrast to the rabbit polyclonal p40, p40 [BC28] does not stain macrophages.

p40 (P) is also available as part of the promotion.

For product information click on the link below to Biocare Medical’s website:

https://biocare.net/product/p40-antibody/

Item No.	Description	*Promo Price (AUD)**
BIC-ACI3066A	p40 (M) 0.1ml Concentrate	$410.00
BIC-ACI3066C	p40 (M) 1ml Concentrate	$1,500.00
BIC-API3066AA	p40 (M) 6ml Predilute	$580.00
BIC-API3066H	p40 (M) 25ml Predilute	$2,040.00
BIC-AVI3066KG	p40 (M) 6ml – Ultraline	$610.00
BIC-ACI3030A	p40 (p) 0.1ml Concentrate	$250.00
BIC-ACI3030B	p40 (p) 0.5ml Concentrate	$710.00
BIC-API3030AA	p40 (p) 6ml Predilute	$340.00

* All pricing excludes freight and GST. Offer available on orders placed from 20 January to 20 March 2021. No additional discounts applied.

METAGENE PROMO2020 discounts run each month (from 20th of Month). Keep checking our website for future promotions!

Terms & Conditions Apply.

INTERSCIENCE – FLEXIPUMP PRO $4,999*

FlexiPump Pro

Dispensing pump for culture media preparation

High-tech and traceability

Cat No. 562 000

FlexiPump Pro is a peristaltic pump with enhanced accuracy for the dispensing of culture media, agar and any type of diluent. Compact and ergonomic, it comes with a user-friendly interface. Traceability is guaranteed by printer and USB connection.

Dispensing mode: Continuous – Dose – Multi-dose
Dose volume: from 50 µL to 99.9 L
Flow rate: up to 2.5 L/mn
Connection: USB, RS232, Jack 3.5 mm

FlexiPump® Pro is a peristaltic pump with enhanced accuracy for the dispensing of culture media, agar and any type of diluent from 50 µL à 99 L. Compact and ergonomic, it comes with a user-friendly interface. Traceability is guaranteed by printer and USB connexion.

Designed for laboratories

FlexiPump is compact and easy to use with 3 dispensing modes: continuous, dose (predetermined volume) and multi-dose (predetermined volume, with an adjustable pause between each dose). It has 24 customizable programs. FlexiPump easily calibrates and picks up in all types of containers.

TECHNICAL SPECIFICATIONS
• Stainless steel
• Watson Marlow™ low pulsation double pump head with 6 rollers
• Dose volume: 50 µL to 99 L
• Dispensing modes: Continuous – Dose – Multi-dose
• Dispensing control with the handy gun
• Multi-dose pause: Adjustable from 0.1 to 99.9 s
• Typical precision with inner Ø 3.2 mm tubing: 9 mL: 0.9% – 18 mL: 0.5% – 225 mL: 0.4%
• Tubing inner diameter: 1.6 mm, 3.2 mm, 4.8 mm, 6.4 mm, 8 mm
• Tubing wall thickness: 1.6 mm only
• Tubings: TygonTM (for pump head) and silicon
• Flow rate: 3 mL/min to 2.5 L/min
• Pump head rotating speed: 10 to 550 rpm
• Traceability: USB, RS 232
• Connections: USB, RS 232, Jack 3.5 mm
• Data export: ExcelTM, Open Office CalcTM, LIMS
• Voltage and frequency: 100-240V∼ 50-60Hz
• IP: 31
• Fuses: T 2.5A L 250V 5×20 mm
• Max power: 50 W
• Dimensions (w x d x h): 16 x 32 x 21 cm, weight: 5 kg
• Box (w x d x h): 50 x 40 x 40 cm, weight: 8 kg
• 1 year warranty
• In compliance with ISO 7218 and FDA BAM (Bacteriological Analytical Manual)
• Manufatured under CE, RoHS, WEEE
• Designed and made in France

Download the brochure: https://www.interscience.com/IMG/pdf/interscience_flexipumppro_ft_en.pdf

Check out the Video:

Or for more information visit: https://www.interscience.com/flexipump-pro-dispensing-pump\

* $4,999.00 pricing excludes freight and GST. Offer available on orders placed from 20 NOVEMBER to 20 DECEMBER 2020.

METAGENE PROMO2020 discounts run each month (from 20th of Month). Keep checking our website for future promotions!

Terms & Conditions Apply.

Go FISH-ing with us! – 20% discount off FISH Probes

FISH (Fluorescence In-Situ Hybridization) probes available include:

Control probes are used for both chromosome enumeration and verifying that copy number variation probes have hybridized to the correct chromosome. Fewer or greater than two signals (one for each sister chromosome) will indicate chromosome aneuploidy.

Break apart probes are used to detect gene translocations. They’re composed of two probes, each bordering one end of the gene. If the gene has rearranged from its typical location, the two probes will split, appearing as two unique signals.

Copy number variation probes are used to detect gene amplifications and deletions. They’re made of one probe designed to hybridize to a single gene. Extra or missing signals will indicate gene gains or losses.

Fusion probes are used to detect fusions between two typically separate genes. They’re made of two probes, each designed to hybridize to its respective gene. If the genes have fused, the probes will appear as one unified signal.

Contact us for more details or a quote today!

or download a brochure:

https://biocare.net/wp-content/uploads/FISHORG101.pdf

* 20% discount is off the full list price of FISH Probes.
Pricing excludes freight and GST. Offer available on orders placed from 20 OCTOBER to 20 NOVEMBER 2020.

METAGENE PROMO2020 discounts run each month (from 20th of Month). Keep checking our website for future promotions!

Terms & Conditions Apply.

Discounted Decloaking Chamber™ NxGen

Decloaking Chamber™ NxGen $5,950*

Engineered pressure chamber for reliable retrieval
HIER buffers with color changing pH indicators
Data recording of pressure, temperature & time

… your complete HIER Quality Control system

Biocare Medical’s Decloaking Chamber is an excellent tool for HIER. The proper use of heat and pressure in conjunction with the appropriate buffer solutions is of the utmost importance for consistent immunohistochemistry (IHC) staining. The Decloaking Chamber is designed to optimize and standardize antibody staining procedures and has been engineered to pass strict laboratory safety and quality control requirements. Temperature, pressure and time can be monitored and recorded to produce consistent staining.

Easy to program
Informative digital display
5 discrete temperature settings
Reproducible results
USB drive data collection
Supports IHC and RISH™ workflow

Optional HIER Buffers

Assure™ color-coded pH indicator technology
Room temperature storage (except EDTA)
Prevent loss of pH sensitive antigens

Product	Composition	pH
Diva Decloaker	Proprietary	6.2
Rodent Decloaker	Proprietary	6.0
Reveal Decloaker	Citrate-based	6.0
Borg Decloaker	Tris-based	9.5
Bull’s Eye Decloaker	Proprietary	6.0
EDTA Decloaker	EDTA-based	8.5
Antigen Decloaker	Citrate-based	6.0
Nuclear Decloaker	Tris-based	9.5

Download the flyer: https://biocare.net/wp-content/uploads/DC12103.pdf

Contact us for a quote today!

* Pricing excludes freight and GST. Offer available on orders placed from 20 SEPTEMBER to 20 DECEMBER 2020.

METAGENE PROMO2020 discounts run each month (from 20th of Month). Keep checking our website for future promotions!

Terms & Conditions Apply.

The Cleanest SOX – 25% Cheaper!

SOX10 (M) [BC34] from Biocare Medical is an exclusive IVD mouse monoclonal antibody that provides a highly specific and sensitive antibody for immunohistochemistry and is virtually background free. We are offering a 25% discount off the full list price of the below Biocare Medical SOX10 products from 20 August to 20 September 2020:

0.1ml SOX10 (M) Concentrate – ACI3099A

1ml SOX10 (M) Concentrate – ACI3099C

6ml SOX10 (M) Predilute – API3099AA

25ml SOX10 (M) Predilute – API3099H

6ml SOX10 (M) Predilute – Ventana – AVI3099G

25ml SOX10 (M) Predilute – Ventana – AVI3099H

For more information see: https://biocare.net/product/sox10-antibody/

Contact us for a quote today!

Discount will be applied to full list price purchases only.
Offer available on orders placed from 20 AUGUST to 20 SEPTEMBER 2020.

METAGENE PROMO2020 discounts run each month (20th of Month to 20th of NextMonth). Keep checking our website for future promotions!

Terms & Conditions Apply.

17 Aug, 2020
(0) Comments
By metagene
Antibodies, Biocare Medical, Products, Sale

COVID-19 Lockdowns – Melbourne & Victoria

MetaGene would like to assure our Melbourne and Regional Victoria customers that we remain open for business and will continue to supply products throughout the COVID-19 lockdowns.

Our main warehouse is located in Queensland and is fully operational.

To date our overseas manufacturers (including Biocare Medical, Horizon Discovery, Santa Cruz Biotechnology, Applied Spectral Imaging, Interscience and Ultivue) as well as our supply chain partners including FedEx, DHL and StarTrack have continued to maintain operations.

We are experiencing some delivery delays and appreciate your patience at this time.

5 Aug, 2020
(0) Comments
By metagene
COVID-19, Lockdowns, Products, Suppliers

Advanced understanding of cancer progression in circulating tumor cells using multiplexed imaging technology

Applied Spectral Imaging (Israel), MetaGene and QIMR Berghofer Medical Research Institute (Australia) have developed a multiplexed fluorescent imaging system to advance the understanding of disease progression in tissue, liquid biopsy and circulating tumor cells. The system offers a cost-effective, rapid, integrated solution for up to 24-plex* MIF analysis.

Original Research Article:

Targeting Nuclear LSD1 to Reprogram Cancer Cells and Reinvigorate Exhausted T Cells via a Novel LSD1-EOMES Switch
Front. Immunol., 16 June 2020 | https://doi.org/10.3389/fimmu.2020.01228

Contact us on 1800 788 498 or admin@metagene.com.au for more information on Applied Spectral Imaging technology, or visit https://spectral-imaging.com/

*Dependent on Filter and Module selection.

23 Jul, 2020
(0) Comments
By metagene
ASI, Cancers, Digital Pathology, GenASIs System, Products