IHC Detection Kits

MACH 4™

MACH 4 is a highly sensitive universal detection system. The MACH 4 system can detect either mouse or rabbit antibodies, and is a two-step universal detection method. MACH 4 is available in a universal (anti-mouse and anti-rabbit) format, labelled with either Horseradish Peroxidase (HRP) or Alkaline Phosphatase (AP). MACH 4 detection consists of two reagents: the secondary, also known as the enhancing reagent, is applied between the primary antibody and micro-polymer reagent. MACH 4 is ideal for use with antibody solutions prepared from concentrates or prediluted antibody solutions. Due to its increased sensitivity, MACH 4 enables a significantly higher dilution of concentrated antibodies compared to other polymer-based detection systems.

MACH 4 MICRO-POLYMER SYSTEM INCORPORATES TWO KEY TECHNOLOGIES:
• Enhanced probe to increase sensitivity 10- to 20- fold
• Increased density of enzymes bound to tertiaries
• 10 – 20 times more sensitive than conventional dextran polymer systems
• 20 – 40 times more sensitive for nuclear staining
• Suitable to detect mouse specific anti-biotin or antidigoxigenin antibody in in situ hybridisation (ISH)
• Micro-polymer allows superior specificity/sensitivity, nuclear access and primary antibody dilution
• Compatible with automated immunostainers
• Compact structure enables 40-fold increase in primary antibody dilution.

COMPARE MACH 4 (L) WITH LEADING COMPETITOR (R) FOR ER (1D5) STAINING

IHC Detection Kits,Mach 4, MetaGene Pty Ltd

MACH 3™

MACH 3 is a two-step, biotin-free detection system which provides excellent specificity, sensitivity and nuclear staining for mouse or rabbit primary antibodies. Biocare offers MACH 3 Detection Kits for either mouse or rabbit primary antibodies, labeled with either Horseradish Peroxidase (HRP) or Alkaline Phosphatase (AP).

MACH 3 FEATURES
• A sensitivity-enhancing secondary reagent
• Increased density of enzymes bound to tertiary target
• 5- to 10- fold increased staining sensitivity when compared to conventional dextran polymer detection kits
• Superior staining for nuclear and cytoplasmic/cell surface antigens
• Elimination of background
• High primary antibody dilution significantly improves specificity and reduces cost
• Adapted for all Biocare’s prediluted antibodies
• Compatible with automated immunostainers
• MACH 3’s superior sensitivity allows clean and strong staining, even at higher primary antibody dilution factors.

COMPARE MACH 3 (LEFT) WITH LEADING COMPETITOR (RIGHT) FOR KI-67

IHC Detection Kits,Mach 3, MetaGene Pty Ltd

MACH 2™

MACH 2 Detection is a one-step / one-solution, biotin-free detection system which combines superior work flow and sensitivity for mouse and rabbit primary antibodies.

MACH 2 detection consists of a single reagent applied after the primary antibody. MACH 2 is ideal for use with prediluted antibodies or concentrates with equal success. MACH 2 may be used for 7-step multiplex stains with certain antibodies.

MACH 2 increases staining specificity compared to conventional dextran backbone polymer. MACH 2 is available in anti-mouse, anti-rabbit, and ‘universal’ (anti-mouse & anti-rabbit) formulations, labeled with either Horseradish Peroxidase (HRP) or Alkaline Phosphatase (AP).

• Increase specificity 3-4 fold in nuclear staining & 2 fold in cytoplasmic/cell surface staining
• Superior to dextran backbone polymers

COMPARE MACH 2 (L) WITH CONVENTIONAL 3-STEP AVIDIN-BIOTIN / ABC SYSTEM (R)

IHC Detection Kits,Mach 2, MetaGene Pty Ltd

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